{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"The Calcium Dependence of  Glycopolymer-Induced in Vitro Acrosome Reaction","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/78235"},{"label":"dc.language.iso","value":"en_US"},{"label":"dcterms.abstract","value":"Sperm acrosomal exocytosis plays an important role in mammalian fertilization. This exocytosis process can be triggered by a subset of terminal carbohydrate residues on the zona pellucida around the mouse egg. Although acrosomal exocytosis (AE) has been successfully induced in vitro using purified mouse zona pellucida protein, the mechanism of AE is unclear.  Previous studies in the Sampson laboratory used a series of glycopolymers that mimic the sugars displayed by the zona pellucida and applied these polymers in an in vitro acrosome assay. The Ca2+-selective ion channel subunits (CatSper) were thought to be the essential ion channel for regulating Ca2+ influx, and intracellular Ca2+ is a crucial factor for successful AE. To understand the Ca2+ dependence of the glycopolymer-induced in vitro AE, we applied a triple stain strategy and flow cytometry technique to test the Ca2+ dependence in the presence and absence of EGTA (ethylene glycol tetraacetic acid).  Three glycopolymers [poly-(mannose)100, poly-(fucose)100 and poly-(glcNAc)100] show clear Ca2+ dependence."},{"label":"dcterms.available","value":"2018-06-21T13:38:39Z"},{"label":"dcterms.contributor","value":"Laughlin, Scott T"},{"label":"dcterms.creator","value":"Zhang, Ye"},{"label":"dcterms.dateAccepted","value":"2018-06-21T13:38:39Z"},{"label":"dcterms.dateSubmitted","value":"2018-06-21T13:38:39Z"},{"label":"dcterms.description","value":"Department of Chemistry"},{"label":"dcterms.extent","value":"94 pg."},{"label":"dcterms.format","value":"Monograph"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/78235"},{"label":"dcterms.issued","value":"2017-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2018-06-21T13:38:39Z (GMT). No. of bitstreams: 1\nZhang_grad.sunysb_0771M_13617.pdf: 7246385 bytes, checksum: 0e2fdddbac949c87ef21e9aa04be323c (MD5)\n  Previous issue date:   12"},{"label":"dcterms.subject","value":"Chemistry"},{"label":"dcterms.title","value":"The Calcium Dependence of  Glycopolymer-Induced in Vitro Acrosome Reaction"},{"label":"dcterms.type","value":"Thesis"},{"label":"dc.type","value":"Thesis"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/76%2F04%2F16%2F76041685484308144076328362353769483543/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/76%2F04%2F16%2F76041685484308144076328362353769483543","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}