{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Identification of Stem Cell Markers in the model organism Nematostella vectensis","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/78222"},{"label":"dc.language.iso","value":"en_US"},{"label":"dcterms.abstract","value":"Nematostella vectensis possess a robust regenerative ability that is characteristic of other cnidarians and highly regenerative model organisms that use stem cells to rebuild missing parts. By identifying somatic stem cell populations and their relationship with regeneration, breakthroughs in regenerative medicine can be made. This study focused on several stem cell marker candidates, Cniwi1, Cniwi2, FoxO and Lin28, as their roles have been associated with stem cell regulation, renewal, and cellular processes. Documenting the spatial and temporal expression patterns of these candidate genes will help validate their functions as stem cell markers. The present study has found that FoxO and Lin28 share similar expression patterns, where expression increases in the later developmental stages: planula and polyp. The expression is localized to the body columns endodermal layer and the aboral region of the pharynx. Cniwi1 and Cniwi2's expression profiles were inconsistent and thus unable to identify any patterning. A reevaluation of the probes may provide a solution to this issue. Additional trials will need to be performed in order to further validate these results and thus identify stem cell markers to be used to study mechanisms of regeneration."},{"label":"dcterms.available","value":"2018-06-21T13:38:35Z"},{"label":"dcterms.contributor","value":"Matus, David Q."},{"label":"dcterms.creator","value":"Cruz Gutierrez, Esau"},{"label":"dcterms.dateAccepted","value":"2018-06-21T13:38:35Z"},{"label":"dcterms.dateSubmitted","value":"2018-06-21T13:38:35Z"},{"label":"dcterms.description","value":"Department of Biochemistry and Cell Biology"},{"label":"dcterms.extent","value":"38 pg."},{"label":"dcterms.format","value":"Application/PDF"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/78222"},{"label":"dcterms.issued","value":"2017-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2018-06-21T13:38:35Z (GMT). No. of bitstreams: 1\nCruzGutierrez_grad.sunysb_0771M_13595.pdf: 1203557 bytes, checksum: 5b51bd72294bde38b8689bd04ddc4a8a (MD5)\n  Previous issue date:   12"},{"label":"dcterms.subject","value":"Piwi"},{"label":"dcterms.title","value":"Identification of Stem Cell Markers in the model organism Nematostella vectensis"},{"label":"dcterms.type","value":"Thesis"},{"label":"dc.type","value":"Thesis"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/43%2F19%2F14%2F43191461234011956983653506559964041283/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/43%2F19%2F14%2F43191461234011956983653506559964041283","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}