{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Biocompatibility and Bioactivity of One- and Two-Dimensional Nanoparticle Reinforced Polymeric Scaffolds for Bone Tissue Engineering Applications","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/76972"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"Limitations associated with current bone grafting techniques utilizing autologous or allogeneic bone have led to an increase in bone tissue engineering strategies. The ideal scheme of repairing a bone defect is to employ a material that can act as a scaffold and assist regeneration of native tissue in the area. Although biodegradable polymers fulfill many of the requirements for synthetic bone grafts, a major limitation is poor integrity under load bearing conditions prompting investigation of nanoparticle-reinforced biodegradable polymer nanocomposites. Recently, incorporation of 0.2 wt% of two-dimensional organic nanostructures (graphene nanoribbons (GONRs) and graphene nanoplatelets (GONPs)) and one- and two-dimensional inorganic nanostructures (molybdenum disulfide nanoplatelets (MSNPs) and tungsten disulfide nanotubes (WSNTs) led to significant enhancement in the compressive modulus, compressive yield strength, flexural modulus, and flexural yield strength of poly(propylene fumarate) (PPF) nanocomposites with when compared to PPF alone or PPF reinforced with single- or multi-walled carbon nanotubes (SWCNTs or MWCNTs). In this work we investigate the cytocompatibility of inorganic nanoparticles to mesenchymal stem cells. We present the in vitro bioactivity of these nanocomposite scaffolds through submersion in simulated body fluid (SBF). From this and previous studies, we select two candidate scaffolds to investigate the in vivo biocompatibility after implantation in the soft and hard tissue of a rat model. Our results indicate little cytotoxicity of the inorganic nanoparticles at concentrations relevant to these scaffolds. We also observed nanoparticle morphology and composition related influence on bioactivity of these nanocomposite scaffolds allowing us to select GONP and MSNP reinforced scaffolds for in vivo investigation. Finally, we observed no deleterious effects from implanting these nanocomposite scaffolds in soft or hard tissue of a rat. The results suggest potential for these nanocomposite scaffolds to serve as bioactive bone tissue engineering scaffolds."},{"label":"dcterms.available","value":"2017-09-20T16:51:34Z"},{"label":"dcterms.contributor","value":"Judex, Stefan"},{"label":"dcterms.creator","value":"Rashkow, Jason Thomas"},{"label":"dcterms.dateAccepted","value":"2017-09-20T16:51:34Z"},{"label":"dcterms.dateSubmitted","value":"2017-09-20T16:51:34Z"},{"label":"dcterms.description","value":"Department of Biomedical Engineering"},{"label":"dcterms.extent","value":"156 pg."},{"label":"dcterms.format","value":"Monograph"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/76972"},{"label":"dcterms.issued","value":"2016-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2017-09-20T16:51:34Z (GMT). No. of bitstreams: 1\nRashkow_grad.sunysb_0771E_12964.pdf: 6199209 bytes, checksum: 35f102e662dd9d78544c3f6c0244f3fa (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"Bioactivity, Biocompatibility, Bone Tissue Engineering, Cytocompatibility, Nanoparticles, Scaffolds"},{"label":"dcterms.title","value":"Biocompatibility and Bioactivity of One- and Two-Dimensional Nanoparticle Reinforced Polymeric Scaffolds for Bone Tissue Engineering Applications"},{"label":"dcterms.type","value":"Dissertation"},{"label":"dc.type","value":"Dissertation"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/39%2F45%2F26%2F39452695514636285845834974588870085995/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/39%2F45%2F26%2F39452695514636285845834974588870085995","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}