{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Alterations to the unique DNA binding mode mediated by MTERF1 have implications of pathogenesis in mitochondrial disease","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/76952"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"Human mitochondria are found in all eukaryotic cells.  They are dynamic double membrane organelles that can vary in shape, size and amount depending on cell types.  In humans, mitochondria contain a 16.5 kb genome that encodes for 22 tRNAs, two rRNAs and 13 proteins that make up the mitochondrial contribution of the oxidative phosphorylation system (OXPHOS).  This system produces the majority of energy used by the cell in the form of ATP and is critical for cell viability.  In order for the OXPHOS system to produce ATP, it is dependent on the proper expression of the mitochondrial genome.  Thus, errors in gene expression can lead to defects in OXPHOS and ultimately pathogenesis of mitochondrial disease.  Therefore, it is important to understand the mechanisms of mitochondrial gene expression and how alteration of these mechanisms can result in pathogenesis.  The initial stage in gene expression is transcription, a process that involves several events including initiation, elongation and termination, all of which are regulated by proteins that are produced in the nucleus and transported to the mitochondria.  Interestingly, termination is one of the least understood aspects of transcription.  It has been established that termination is regulated by the mitochondrial transcription termination factor MTERF1.  Our lab has solved the crystal structure of MTERF1 bound to its canonical DNA sequence located within the tRNA<super>Leu</super> gene and made great progress in understanding the mechanisms critical for MTERF1 mediated transcription termination.  Most strikingly, MTERF1 exhibits a unique DNA binding mode that involves the formation of sequence specific interactions, helix unwinding and the eversion of three nucleotides stabilized by stacking interactions outside the double helix.  Further structural and biochemical characterization of the base flipping mechanics has revealed a stepwise order to the base-flipping event that is important for function.  In addition, we show that pathogenic mutations present within the DNA binding site perturb sequence recognition and base flipping.  These result in termination defects that have implication for pathogenesis of mitochondrial disease."},{"label":"dcterms.available","value":"2017-09-20T16:51:31Z"},{"label":"dcterms.contributor","value":"Seeliger, Markus"},{"label":"dcterms.creator","value":"Byrnes, James"},{"label":"dcterms.dateAccepted","value":"2017-09-20T16:51:31Z"},{"label":"dcterms.dateSubmitted","value":"2017-09-20T16:51:31Z"},{"label":"dcterms.description","value":"Department of Biochemistry and Structural Biology."},{"label":"dcterms.extent","value":"119 pg."},{"label":"dcterms.format","value":"Application/PDF"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/76952"},{"label":"dcterms.issued","value":"2014-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2017-09-20T16:51:31Z (GMT). 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