{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"The role of the Kin28 protein kinase in chromatin remodeling and transcription termination","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/76892"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"The Kin28 subunit of the general transcription factor complex, TFIIH, is a kinase that phosphorylates the heptapeptide repeats at serine position 5 (Ser5) on the C-terminal domain of RNA polymerase II (Pol II). Optimal Ser5 phosphorylation is important during transcription for Pol II to transition from the initiation step to the elongation step in a process called promoter escape. During transcription initiation, the nucleosome directly downstream of the promoter, called +1, must be disassembled before Pol II can engage the transcription start site. Previous work showed that the transcription machinery plays a direct role in the disassembly of the +1 nucleosome, but the exact mechanism is currently unknown. We hypothesize that the disassembly of the +1 nucleosome occurs during promoter escape. To test this idea, I attempted to conditionally deplete Kin28, in the yeast, Saccharomyces cerevisiae, to block promoter escape. I then applied a modified genomic approach to map nascent Pol II-associated RNA molecules before and after Kin28 depletion. The Kin28 depletion failed to block promoter escape, possibly due to incomplete removal of Kin28 from the nucleus. Instead, I found that the Kin28 depleted strain accumulated Pol II-associated read-through transcripts beyond the transcription termination site suggesting that Kin28 plays a role in the processing of the nascent RNA of protein-coding genes."},{"label":"dcterms.available","value":"2017-09-20T16:51:23Z"},{"label":"dcterms.contributor","value":"Luk, Ed"},{"label":"dcterms.creator","value":"Labuz, Daniel Roy"},{"label":"dcterms.dateAccepted","value":"2017-09-20T16:51:23Z"},{"label":"dcterms.dateSubmitted","value":"2017-09-20T16:51:23Z"},{"label":"dcterms.description","value":"Department of Biochemistry and Cell Biology"},{"label":"dcterms.extent","value":"49 pg."},{"label":"dcterms.format","value":"Monograph"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/76892"},{"label":"dcterms.issued","value":"2016-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2017-09-20T16:51:23Z (GMT). No. of bitstreams: 1\nLabuz_grad.sunysb_0771M_13119.pdf: 3574928 bytes, checksum: 84a7cd55029d3655d59b938d7d5602c2 (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"Biochemistry -- Molecular biology"},{"label":"dcterms.title","value":"The role of the Kin28 protein kinase in chromatin remodeling and transcription termination"},{"label":"dcterms.type","value":"Thesis"},{"label":"dc.type","value":"Thesis"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/22%2F90%2F81%2F22908116681833222439174856661790727954/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/22%2F90%2F81%2F22908116681833222439174856661790727954","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}