{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Effect of Toxicity of RDP in Polymer Scaffolds on Fibroblast Cell","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/76363"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"The purpose of this thesis is to design substrates with flame retardant and clay to enhance the property of polymer and to study the effect of toxicity of a flame retardant mixed polymer, which had not been conducted in vivo. Therefore the human dermal fibroblast cells, engineered to have green fluorescent membrane (GFFB), were cultured on PS scaffolds with three different conditions: pure PS, PS-Na+-Clay and RDP-Clay. In first part of the study, the polymer samples (PS, PS-Clay and RDP-clay) that would be used as scaffolds were prepared and sterilized to use for cell culture, while testing that sterilization using autoclave was adaptive in the experiment. SEM, EDX spectroscopy and contact angle measurement were used to see if there was any change in the polymers after sterilized in autoclave. In second part of the study, the prepared GFFB cells were cultured on the substrates used as scaffolds and the cell growth was observed by cell counting using both hemocytometer and observation under a fluorescent microscopy. The condition of cells was also observed using a confocal microscopy. Results showed that cell proliferation rates were different for each type polymer scaffolds, and surprisingly, RDP-Clay PS polymer showed the highest cell proliferation rate and lowest doubling time, similar to the control group, which used a commercial PS tissue culture plate. It was determined that RDP was not toxic, but helpful for cell growth. Therefore further study might be needed to figure out the mechanism of RDP effects on the cell growth."},{"label":"dcterms.available","value":"2017-09-20T16:50:06Z"},{"label":"dcterms.contributor","value":"Kim, Tae Jin."},{"label":"dcterms.creator","value":"Yang, Jae Wha"},{"label":"dcterms.dateAccepted","value":"2017-09-20T16:50:06Z"},{"label":"dcterms.dateSubmitted","value":"2017-09-20T16:50:06Z"},{"label":"dcterms.description","value":"Department of Materials Science and Engineering."},{"label":"dcterms.extent","value":"77 pg."},{"label":"dcterms.format","value":"Application/PDF"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/76363"},{"label":"dcterms.issued","value":"2015-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2017-09-20T16:50:06Z (GMT). No. of bitstreams: 1\nYang_grad.sunysb_0771M_12636.pdf: 7441234 bytes, checksum: bb8ff4858d5880388faddc0971310251 (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"Materials Science"},{"label":"dcterms.title","value":"Effect of Toxicity of RDP in Polymer Scaffolds on Fibroblast Cell"},{"label":"dcterms.type","value":"Thesis"},{"label":"dc.type","value":"Thesis"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/11%2F53%2F66%2F115366090154914414751386886375187764534/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/11%2F53%2F66%2F115366090154914414751386886375187764534","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}