{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Novel Carbon Nanomaterial Coating \nfor Dispersibility, Delivery and Sensing","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/71428"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"Carbon nanomaterials have been cited to provide great potential in \nbiomedical applications such as in vivo imaging, drug delivery, and biomarker detection. Yet \npoor dispersibility in physiological conditions greatly limits their biomedical promise. As \nwith most nanoparticles, the surface interaction with biological systems is the driving force \ntowards effective activity in vivo, namely exhibiting dispersion, low cytotoxicity, and \nmolecular targetability. Therefore, by surface engineering carbon nanomaterials with a \ndistinct biocompatible coating, their applications in imaging, drug delivery, biomarker \ndetection, and therapy can be empowered. We render carbon nanomaterials useful for such in \nvivo biomedical applications by providing dispersibility, delivery and sensing capabilities \nwith a facile surface coating method. A single, yet multifunctional, hyaluronic acid-based \nbiosurfactant was strategically chosen to meet the design criteria. The amphiphilic material, \nhyaluronic acid-5\u0392-cholanic acid (HACA), is an efficient dispersing agent for carbon \nnanomaterials, including single-walled carbon nanotubes (SWCNTs), in physiological conditions \nfor a sustained period of time. Furthermore, the biological activity and cancer cell \ntargeting of HACA wrapped SWCNTs (HACA-SWCNTs) were evaluated in vitro and in vivo utilizing \nimaging techniques intrinsic to SWCNTs, HACA, and HACA-SWCNTs. Fluorescent dye-labeled \nHACA-SWCNTs were designed to activate fluorescence signals intracelluarly, not only serving \nas an approach to image cellular uptake but also to determine the coating efficacy of HACA \nonto SWCNTs. SWCNT localization within cells was also confirmed by tracking the intrinsic \nRaman signals of carbon nanomaterials. In vivo photoacoustic, fluorescence, and positron \nemission tomography imaging display high tumor targeting capability of HACA-SWCNTs in a \nmurine tumor model. Once targeted, HACA-SWCNTs have potential to serve as photothermal tumor \nablation agents after laser activation. HACA coating of carbon nanomaterials creates a system \nto simultaneously 1) disperse insoluble carbon-based materials, 2) target these coated \nmaterials to cancer cells, 3) image intracellular uptake of the platform in vitro and in vivo \nand, after integrating these properties, 4) serve as therapeutics. This work brings carbon \nnanomaterials closer to their biomedical potential."},{"label":"dcterms.available","value":"2015-04-24T14:47:32Z"},{"label":"dcterms.contributor","value":"Frame, Mary D"},{"label":"dcterms.creator","value":"Swierczewska, Magdalena"},{"label":"dcterms.dateAccepted","value":"2013-05-22T17:35:41Z"},{"label":"dcterms.dateSubmitted","value":"2013-05-22T17:35:41Z"},{"label":"dcterms.description","value":"Department of Biomedical Engineering"},{"label":"dcterms.extent","value":"137 pg."},{"label":"dcterms.format","value":"Application/PDF"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/11401/71428"},{"label":"dcterms.issued","value":"2012-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2013-05-22T17:35:41Z (GMT). No. of bitstreams: 1\nSwierczewska_grad.sunysb_0771E_11200.pdf: 7016467 bytes, checksum: 83f9938ae9000179a1be95057b47582e (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"Nanotechnology--Biomedical engineering--Materials Science"},{"label":"dcterms.title","value":"Novel Carbon Nanomaterial Coating \nfor Dispersibility, Delivery and Sensing"},{"label":"dcterms.type","value":"Dissertation"},{"label":"dc.type","value":"Dissertation"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/48%2F35%2F49%2F48354902725161847748033428244594001989/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/48%2F35%2F49%2F48354902725161847748033428244594001989","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}