{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Use of Native DNA Binding Domains Tethered to the Fok1 Endonuclease to Target Nodal Related Genes via the Activin Response Element","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/11401/71346"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"Use of zinc finger nucleases is an established method for gene targeting in zebrafish.  Such strategies are efficient in targeting a single gene of interest.  Our study attempts to target many Nodal related genes by targeting the Activin Response Element (ARE) through the use of native DNA binding domains tethered to the Fok1 endonuclease.  In this light, genes are targeted in a Nodal-dependent manner, and can retain transcript levels sustained by other signaling pathways.  This technique holds the potential to be a high throughput means of generating mutations in the regulatory regions of various Nodal responsive genes with the use of one set of constructs."},{"label":"dcterms.available","value":"2015-04-24T14:47:08Z"},{"label":"dcterms.contributor","value":"Sirotkin, Howard I"},{"label":"dcterms.creator","value":"Minder, Jessica Lauren"},{"label":"dcterms.dateAccepted","value":"2013-05-22T17:35:16Z"},{"label":"dcterms.dateSubmitted","value":"2013-05-22T17:35:16Z"},{"label":"dcterms.description","value":"Department of Biochemistry and Cell Biology"},{"label":"dcterms.extent","value":"59 pg."},{"label":"dcterms.format","value":"Monograph"},{"label":"dcterms.identifier","value":"Minder_grad.sunysb_0771M_11202"},{"label":"dcterms.issued","value":"2012-12-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2013-05-22T17:35:16Z (GMT). No. of bitstreams: 1\nMinder_grad.sunysb_0771M_11202.pdf: 8448813 bytes, checksum: 35f9c19b1374ebeb37aef12c9d80f73b (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"Activin Response Element, Gene Targeting, Nodal"},{"label":"dcterms.title","value":"Use of Native DNA Binding Domains Tethered to the Fok1 Endonuclease to Target Nodal Related Genes via the Activin Response Element"},{"label":"dcterms.type","value":"Thesis"},{"label":"dc.type","value":"Thesis"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/14%2F47%2F00%2F144700447130621333123958871711659789904/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/14%2F47%2F00%2F144700447130621333123958871711659789904","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}