{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Nitric oxide regulation of c-di-GMP metabolism and biofilm formation in Shewanella woodyi","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/1951/59766"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"Biofilms, multi-cellular sessile communities of bacteria, are known to account for bacterial persistence and antibiotic resistance. Nitric oxide (NO) has been shown to induce biofilm dispersal at sub-lethal concentrations in many species. For example, in the cystic-fibrosis associated bacterium, Pseudomonas aeruginosa, NO is reported to regulate biofilm dispersal through cyclic di-GMP signaling. In this thesis work, I demonstrate that Swoo_2750 from Shewanella woodyi encodes a Heme-Nitric oxide/OXygen binding domain (H-NOX), a protein that binds NO with approximately picomolar sensitivity. I demonstrate further that SwH-NOX is co-cistronic and directly interacts with Swoo_2751, a bi-functional diguanylate cyclase (DGC), which exhibits both c-di-GMP synthesis and hydrolysis activities. Through steady-state kinetic analyses, I conclude that NO bound H-NOX interacts with DGC and induces a 15-fold increase in c-di-GMP hydrolysis as well as a 90% decrease in c-di-GMP synthesis compared to the effect on DGC found with unligated H-NOX. I have correlated these biochemical data with measurements of in vivo c-di-GMP concentrations and assessments of biofilm formation, both in wildtype and a mutant (Δhnox) strain of S. woodyi. These studies lead to the conclusion that this H-NOX signaling pathway provides a molecular-level explanation for the rapid dispersal of biofilms that has been observed in the presence of NO. Finally, I have explored the source of NO used in H-NOX signaling, especially the role of anaerobic respiration in regulation of biofilm formation in S. woodyi. In summary, I present here my effort towards understanding nitric oxide signaling in biofilm regulation."},{"label":"dcterms.available","value":"2015-04-24T14:47:02Z"},{"label":"dcterms.contributor","value":"Seeliger, Jessica C"},{"label":"dcterms.creator","value":"Liu, Niu"},{"label":"dcterms.dateAccepted","value":"2015-04-24T14:47:02Z"},{"label":"dcterms.dateSubmitted","value":"2015-04-24T14:47:02Z"},{"label":"dcterms.description","value":"Department of Chemistry"},{"label":"dcterms.extent","value":"117 pg."},{"label":"dcterms.format","value":"Monograph"},{"label":"dcterms.identifier","value":"Liu_grad.sunysb_0771E_11118"},{"label":"dcterms.issued","value":"2012-08-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2015-04-24T14:47:02Z (GMT). No. of bitstreams: 3\nLiu_grad.sunysb_0771E_11118.pdf.jpg: 1894 bytes, checksum: a6009c46e6ec8251b348085684cba80d (MD5)\nLiu_grad.sunysb_0771E_11118.pdf.txt: 214809 bytes, checksum: e8e3dbc4a64faa90e3317cea7e32b793 (MD5)\nLiu_grad.sunysb_0771E_11118.pdf: 3748612 bytes, checksum: 14d2cde0529209fa091d73bbb4f05339 (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"biofilm, c-di-GMP, diguanylate cyclase, nitric oxide, phosphodiesterase"},{"label":"dcterms.title","value":"Nitric oxide regulation of c-di-GMP metabolism and biofilm formation in Shewanella woodyi"},{"label":"dcterms.type","value":"Dissertation"},{"label":"dc.type","value":"Dissertation"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/54%2F59%2F66%2F54596624359106307783965673128075942879/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/54%2F59%2F66%2F54596624359106307783965673128075942879","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}