{"@context":"http://iiif.io/api/presentation/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/manifest.json","@type":"sc:Manifest","label":"Population genetic structure of Lemon Sharks in the \nWestern Atlantic: is there evidence of gender-biased dispersal and \ndifferences between neutral and adaptive loci?","metadata":[{"label":"dc.description.sponsorship","value":"This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree."},{"label":"dc.format","value":"Monograph"},{"label":"dc.format.medium","value":"Electronic Resource"},{"label":"dc.identifier.uri","value":"http://hdl.handle.net/1951/55950"},{"label":"dc.language.iso","value":"en_US"},{"label":"dc.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.abstract","value":"Sampling difficulties and low genetic diversity \nhave limited population genetic studies of large, vagile shark species.  \nThrough extensive sampling (580 individuals from 12 locations) and a \nmultilocus approach involving two mitochondrial DNA loci (control region \nand ND2, composite sequence 1,730 bp) and eight microsatellites, I was \nable to delineate the population genetic structure of the lemon shark, \nNegaprion brevirostris, in the western Atlantic.  I articulated two \nhypotheses to explain genetic structure in coastal sharks: (1) females \nexhibit natal philopatry to parturition sites, resulting in local \npopulation structure and (2) males are dispersive, resulting in high \nmale-mediated gene flow.  I predicted that maternally inherited \nmitochondrial genetic markers would be highly structured even on a local \ngeographic scale, while bi-parentally inherited microsatellite markers \nwould exhibit little to no genetic structure over the same range. \nSignificant genetic structure was detected in the mitochondrial \ncomposite sequence of ND2 and control region \n(\u03a6<sub>ST</sub> =0.293, p<0.000001), with at least \nseven distinct groups evident in the sampling area (East Peninsular \nFlorida, West Peninsular Florida/Tiger Beach (Bahamas), Lower Florida \nKeys/Bimini (Bahamas)/Belize, Eleuthera (Bahamas), Louisiana, U.S. \nVirgin Islands and Brazil). Significant genetic structure was \nindividually detected in both the coding ND2 \n(\u03a6<sub>ST</sub> =0.293, p<0.000001) and the \nnon-coding control region (\u03a6<sub>ST</sub> =0.278, \np<0.000001), but the ND2 gene was found to be an inappropriate locus \nto test for local adaptation in lemon sharks because all of the \nmutations were silent with the exception of a single mutation found in \ntwo sharks.  Very weak genetic structure was also detected in nuclear \nmicrosatellites (F<sub>ST</sub> =0.014; p<0.088), but \nonly between the Brazilian population and all of the others. A Bayesian \nanalysis of the microsatellite data failed to reject a null hypothesis \nthat there is one population in the region. Both classes of genetic \nmarker indicated that geographic distance between sampling areas was \ncorrelated with genetic distance between them. All of these findings are \nconsistent with my predictions and support the hypotheses of natal \nphilopatry in females and high male-mediated gene flow. Fine-scale local \ngenetic structure driven by behavior makes this species and others like \nit much more vulnerable to local fishing or habitat destruction than \nresource managers currently appreciate. I suggest future work should \nsequence larger regions of the mitochondrial genome to fully resolve \npopulation structure in this species and to investigate the possibility \nof local genetic adaptation in the coding regions."},{"label":"dcterms.available","value":"2012-05-17T12:19:47Z"},{"label":"dcterms.contributor","value":"Demian DF. Chapman.  Committee"},{"label":"dcterms.creator","value":"Ashe, Jimiane \nLee"},{"label":"dcterms.dateAccepted","value":"2015-04-24T14:44:41Z"},{"label":"dcterms.dateSubmitted","value":"2015-04-24T14:44:41Z"},{"label":"dcterms.description","value":"Department of Marine and \nAtmospheric Science"},{"label":"dcterms.format","value":"Monograph"},{"label":"dcterms.identifier","value":"http://hdl.handle.net/1951/55950"},{"label":"dcterms.issued","value":"2011-08-01"},{"label":"dcterms.language","value":"en_US"},{"label":"dcterms.provenance","value":"Made available in DSpace on 2012-05-17T12:19:47Z (GMT). No. of bitstreams: 1\nAshe_grad.sunysb_0771M_10659.pdf: 3996026 bytes, checksum: 05315baaca67637862a069189898587f (MD5)\n  Previous issue date:    1"},{"label":"dcterms.publisher","value":"The Graduate School, Stony Brook University: Stony Brook, NY."},{"label":"dcterms.subject","value":"lemon \nshark, male-biased dispersal, microsatellites, mitochondrial DNA, natal \nphilopatry, population genetics"},{"label":"dcterms.title","value":"Population genetic structure of Lemon Sharks in the \nWestern Atlantic: is there evidence of gender-biased dispersal and \ndifferences between neutral and adaptive loci?"},{"label":"dcterms.type","value":"Thesis"},{"label":"dc.type","value":"Thesis"}],"description":"This manifest was generated dynamically","viewingDirection":"left-to-right","sequences":[{"@type":"sc:Sequence","canvases":[{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json","@type":"sc:Canvas","label":"Page 1","height":1650,"width":1275,"images":[{"@type":"oa:Annotation","motivation":"sc:painting","resource":{"@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/36%2F53%2F05%2F36530556466328559939775158594606608193/full/full/0/default.jpg","@type":"dctypes:Image","format":"image/jpeg","height":1650,"width":1275,"service":{"@context":"http://iiif.io/api/image/2/context.json","@id":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/36%2F53%2F05%2F36530556466328559939775158594606608193","profile":"http://iiif.io/api/image/2/level2.json"}},"on":"https://repo.library.stonybrook.edu/cantaloupe/iiif/2/canvas/page-1.json"}]}]}]}